mmc2021 Conference Sessions
The conference at mmc2021 will consist of six parallel virtual streams comprising 36 conference and EMAG sessions.
The conference sessions will be recorded and available after their live scheduled time for those who registered for mmc2021. The recordings will be made available to view for a few weeks after mmc2021.
You can view the EMAG session keywords on the EMAG 2021 webpage, the remaining conference sessions are listed below. For more information on our Invited Speakers please click on their name.
AFM & Scanning Probe Microscopies Sessions
AFM/SPM: SPM Techniques on Energy Materials and Processes
Session Chair: Professor Ulrich Stimming (Newcastle University, UK)
This session will bring together researchers from the Scanning Probe Microscopy community that are engaged in studying problems relating to energy. All researchers applying techniques, such as AFM, STM, scanning Kelvin probes, SECPM and more to energy related aspects are invited to submit their contributions. It is the goal of the session to demonstrate how the various SPM techniques are able to study materials as used in energy conversion and storage devices. SPM techniques can often be used under in-situ conditions, i.e. under control of the potential in electrochemical systems; this carries clear advantages compared to any vacuum-based characterisation techniques. It would be an important goal of the session to demonstrate the advantages of the in-situ approach.
Dr Wing Chung Tsoi (Swansea University, UK)
Talk title: Multi-functional and in-situ mapping techniques for optoelectronic devices
Dr Wing Chung Tsoi is a Senior Lecturer from Materials Research Centre at the College of Engineering, Swansea University. He obtained his Higher Diploma in Applied Physics and BSc in Physics from The Hong Kong Polytechnic University and The University of Hull, respectively. He then obtained his PhD in Physics at The University of Hull in 2006. After that, he took a postdoctoral position at The University of Sheffield. From 2009, he obtained a EPSRC-NPL postdoctoral partnership with Imperial College and National Physical Laboratory. From 2014, he was awarded Knowledge Transfer Secondment with Imperial College and NPL. During 2014-2019, he was a senior research officer at Swansea University. In 2019, he was a lecturer there and he was subsequently promoted to his current position in 2020. His research interests are mainly focus on solution-process photovoltaic/solar cells, including organic solar cells, perovskite solar cells...He is particularly interested in understanding the structure-property-performance relationship of the photovoltaic/solar cells, in order to understand the fundamentals and to improve the performance, particularly the stability of the photovoltaic/solar cells. His expertise is on developing/applying advanced (nano-) characterization methods to probe the structures/morphology of the photovoltaic thin films, and correlate it to the properties and performances. His particularly expertise is on advanced Raman system-based spectroscopy/mapping and functional atomic force microscopy as well as photovoltaic cells for indoor, power generation window and aerospace applications. Dr Tsoi have published 69 papers and involved in 60 oral presentations in international/national conferences including a number of invited talks.
Dr Wing Chung Tsoi is the winner of the 2021 RMS Medal for Innovation in Applied Microscopy for Engineering and Physical Sciences, this will be presented at mmc2021.
- Talk title: Multi-functional and in-situ mapping techniques for optoelectronic devices
Professor Dr Andreas Bund (Technische Universitaet Ilmenau, Germany)
Talk Title: Characterization of active materials for lithium ion batteries using scanning probe techniques
Andreas Bund is currently the head of the electrochemistry and electroplating group at Technische Universitaet Ilmenau. He studied chemistry at the University of Saarland (Saarbruecken, Germany) and obtained his PhD there in 1999 under the supervision of Guenter Schwitzgebel. Afterwards he joined the group of Waldfried Plieth at Technische Universitaet Dresden as a PostDoc and worked on the fundamentals and applications of electrochemical deposition processes. With a Heisenberg fellowship of the German Research Foundation (DFG) he could join the group of Henry S. White at the University of Utah in 2006 and 2007. There he worked on the electrochemistry of nanopores. From 2009 - 2010 he had a guest professorship at TU Muenchen at the Chair of Interfaces and Energy Conversion, which was headed by Ulrich Stimming at that time. In 2010 he was appointed full professor at TU Ilmenau. For the first five years this was an endowed professorship financially supported by the German association of electroplaters (ZVO). In 2019 he had a guest professorship at Keio University (Yokohama) and worked with Yasushi Katayama on electrochemical processes in ionic liquids. His research interest comprise fundamental and applied electrochemistry, such as electrodeposition and electrochemical storage and conversion of energy.
- Talk Title: Characterization of active materials for lithium ion batteries using scanning probe techniques
AFM/SPM: New Frontiers in Quantum Matter Visualization
Session Chair: Professor Séamus Davis (University of Oxford, UK)
This session will focus on newly developing and highly advanced techniques for atomic-scale visualization of electronic quantum matter. These include scanned Josephson tunneling microscopy, scanned shot-noise microscopy, scanned fano spectroscopy and quantum-phase resolved quasiparticle interference imaging. Areas of application for these techniques include studies of strongly-correlated electron fluids, strongly-correlated superconductors with complex order parameters, and strongly-correlated electronic liquid crystals or electron-pair crystals.
Dr Freek Massee (CNRS, France)
Talk title: Imaging dynamics using atomic scale shot noise
Since 2017 I am a permanent CNRS researcher in the group Nanostructures at the Nanosecond (NS2) at the Laboratoire de Physique des Solides (LPS) in Orsay where I have set up a low temperature scanning probe facility with shot-noise capabilities. With this facility we aim to get a deeper understanding of dynamics and correlations at the atomic scale. Before joining the LPS as a Marie Curie fellow in 2015, I have worked as a postdoc with Seamus Davis at Cornell University (2012-2015) uncovering the gap structure of the heavy Fermion superconductor CeCoIn5, and studying the pairing interaction and vortex pinning of iron based superconductors. My PhD with Mark Golden and Jeroen Goedkoop at the University of Amsterdam (2007-2011) focussed on bilayer colossal magnetoresistant manganites and iron based superconductors using scanning tunnelling microscopy and angle resolved photoemission spectroscopy. My name currently appears on 21 publications which have been cited in total >700 times (H-index = 14), and I have had the pleasure to speak about my work at 31 conferences and seminars (20 invited).
- Talk title: Imaging dynamics using atomic scale shot noise
Dr Hermann Suderow (Universidad Autónoma de Madrid, Spain)
Talk title: Hopf bifurcations in scanning tunneling microscopy
Scanning tunneling microscopy at 100 mK. Determination of the superconducting gap and electronic density of states in more than twenty different compounds. Development and construction of new experiments, such as microscopy in three-axis magnets. Development of the synthesis of new quantum materials at the Universidad Autonoma de Madrid. PhD (1997) on thermal conductivity measurements at very low temperatures (15 mK) in the heavy fermion superconductor UPt3, establishing the quasiparticle excitation spectrum of nodal superconductors. Marie Curie fellow (1998-2000), Ramón y Cajal fellow (2001-2006), Profesor Titular (2006-2019), having passed through the state habilitation contest and Full Prof since 2019. Teacher of advanced laboratory, solid state and statistical physics. Fellow APS 2017. Manager (president of the physics panel) of the Spanish Science State Agency (2015-2018). Director of the Nicolás Cabrera Institute (2011-2020, www.uam.es/inc), coordinator for materials sience at www.uam.es/ifimac, coordination work at segainvex (www.uam.es/segainvex) and member of panels and commissions such as C5 IUPAP. 110 articles, h-index of 26, more than 50 invited contributions to international meetings, including major keynote talks, 2 patents, 7 co-directed PhD thesis, participation in numerous PhD committees. Action Chair of COST project (nanocohybri.eu), PI of 12 projects and co-PI in 2 Marie Curie projects. Co-organizer of 7 international meetings.
- Talk title: Hopf bifurcations in scanning tunneling microscopy
AFM/SPM: Functional Scanning Probe Microscopy for Advanced Material Science
Session Chair: Dr Olga Kazakova (National Physical Laboratory, UK)
This session will bring together researchers in physics, material science, instrumentation, machine learning, computing and beyond, linked by a common interest in the role of SPM to elucidate new physical phenomena. Researchers from industry, academia and research institutions will present their latest developments to foster new ideas and collaborations across multiple disciplines. Submissions covering all forms of SPM techniques, instrumentation and application are welcome, including (but not limited to) SPM for Quantum (incl. magnetic) systems and 2D materials beyond graphene, novel instrumentation and techniques, functional imaging and spectroscopy, advanced data processing, big data, Machine learning, AI in SPM, theory and simulations.
Dr Laura Fumagalli (University of Manchester, UK)
Talk title: Probing the anomalous dielectric properties of interfacial water on the nanoscale
Laura Fumagalli is Reader in Condensed Matter Physics at the Department of Physics & Astronomy of the University of Manchester (UK) and staff researcher of the National Graphene Institute. She graduated in Electronic Engineering in 2002 (Polytechnic University of Milan - Italy and École Superior d’Electricité - France). She received her PhD in Information Technology in 2006 with a doctoral thesis on low-noise widebandwidth amplifiers at the Department of Electronics of Polytechnic University of Milan. She worked as post-doctoral researcher (2006-2010) and then lecturer (2010-2014) at the Department of Electronics of the University of Barcelona (Spain), where she pioneered the development of a novel nanoscopic technique - Scanning Dielectric Microscopy - that allows measuring the dielectric properties of matter on the nanoscale using a scanning probe. She joined the Condensed Matter Physics group of the University of Manchester (UK) in 2015. She was awarded the prestigious Ramon y Cajal fellowship by the Spanish Ministry of Education and Science in 2014 and the ERC-Consolidator grant award ‘Liquid2DM’ by the European Research Council in 2018. Her research focuses on the study of electric and dielectric properties of bio and non-bio materials on the nano- and atomic scale, with particular interest in nanoconfined water and the solidliquid interface, biomembranes and biomolecules (DNA, proteins), van der Waals crystals and their heterostructures.
Dr Laura Fumagalli is the winner of the 2021 RMS Medal for Atomic Force Microscopy and Scanning Probe Microscopy, this will be presented at mmc2021.
- Talk title: Probing the anomalous dielectric properties of interfacial water on the nanoscale
Professor Frank Koppens (ICFO Institute of Photonic Sciences, Spain)
Talk title: Optical near-field microscopy for stacked and twisted 2D materials
Professor Frank Koppens obtained his PhD in experimental physics at Delft University, at the Kavli Institute of Nanoscience, The Netherlands. After a postdoctoral fellowship at Harvard University, Since August 2010, Koppens is group leader at the Institute of Photonic Sciences (ICFO). The quantum nano-optoelectronics group of Professor Koppens focuses on both science and technology of novel two-dimensional materials and quantum materials.
Professor Koppens is vice-chairman of the executive board of the graphene flagship program, a 1000 MillionEuro project for 10 years. He is also the leader of the optoelectronics workpackage within the flagship. Professor Koppens holds a GSMA Chair with activities related to the Mobile World Congress.
Koppens has received five ERC awards: the ERC starting grant, the ERC consolidator grant, and three ERC proof-of-concept grants. Other awards include the Christiaan Hugyensprijs 2012, the national award for research in Spain, the IUPAP young scientist prize in optics, and the ACS photonics investigator award. In total, Koppens has published more than 80 refereed papers (H-index above 50), with more than 35 in Science and Nature family journals. Total number of citations exceeds 20.000 (google scholar). Weblinks: Graphene.icfo.eu, Koppensgroup.icfo.eu
- Talk title: Optical near-field microscopy for stacked and twisted 2D materials
AFM/SPM: Quantitative SPM for Biology, Biomedicine, and Bioinspired Technologies
Session Chair: Professor Brian Rodriguez (University College Dublin, Ireland)
Scanning probe microscopy (SPM) has found wide application in basic and applied biology-related research owing to its capacity for quantitative imaging and spectroscopy of a diverse set of functional properties at the micro- and nanoscale. The high resolution and high force sensitivity of SPM modes and the ability of SPM to operate in physiological conditions, at high speeds, and in conjunction with optical microscopy have made it an indispensable tool, suited to the wide ranging needs of biological research from single proteins to complex heterogeneous materials and biological systems including living cells, tissues, tumours, viruses, bacteria, plants, fungi, etc. The use of SPM for biological research has long been a driving force for application and technique development, and advances in correlative microscopy and multiparametric modes provide opportunities for further contributions in methodology, instrumentation, and analysis. Speakers in this session will showcase how they are using SPM, including structural, nanomechanical, and electrical imaging and spectroscopy modes as well as ion conductance microscopy, to address challenges in biological, biomedical, and bioinspired research.
Dr Laia Pasquina-Lemonche (University of Sheffield, UK)
Talk title: The Gram-positive bacterial cell wall: an evolving heterogeneous hydrogel characterised by AFM
Dr Laia Pasquina-Lemonche is a Research Associate in Professor Jamie Hobbs’ group at University of Sheffield. She graduated from Physics at the Autonomous University of Barcelona (UAB) in 2015. Then, she received a Master in Nanobiotechnology and Nanoscience at UAB in 2016. Then, she started her PhD in Biophysics at the University of Sheffield focused on deciphering the molecular architecture of gram-positive bacterial cell wall using AFM (L.Pasquina-Lemonche, J.Burns, et al, 2020, Nature). After her PhD, she keeps working in the Hobbs group focusing on understanding how the bacterial cell wall structure changes upon antibiotic treatments and other environments. Recently, she is developing new approaches to push the limits of resolution of biological material with AFM with the aim to study the peptidoglycan architecture of the cell wall more in detail.
- Talk title: The Gram-positive bacterial cell wall: an evolving heterogeneous hydrogel characterised by AFM
Dr Pedro J de Pablo (Universidad Autónoma de Madrid, Spain)
Talk title: Physical Virology with Atomic Force Microscopy
Pedro José de Pablo Gómez, was born in Madrid on March 1972. He finished the studies of Physics (Condensed Mattter) at Universidad Autónoma de Madrid on September 1995. He obtained the PhD entitled “Transport properties of mesoscopic systems studied with Atomic Force Microscopy (AFM)” on December 2000, where he studied the electronic transport of molecular wires, such as carbon nanotubes and DNA. He gained the Extraordinary Ph. D. Prize of UAM. In 2001 he moved to The Netherlands to carry on postdoctoral research on single-molecule biophysics and performed studies of molecular motors (kinesin) and bacteriophage phi29 with AFM. In September 2003 Dr. De Pablo started his own research line on investigating the trinomial structure-function-properties of viruses with AFM. Viruses studied include minute virus of mice, bacteriophages phi29, T7, lambda, P22, human adenovirus, rotavirus, etc. He specialized in measuring the physical properties of viruses, such as stiffness, pressure, fatigue, electrostatics, etc. He aims to establish fruitful relationships between these properties and the function of viruses.
- Talk title: Physical Virology with Atomic Force Microscopy
Frontiers in Bioimaging Sessions
Frontiers in Bioimaging: Development and Applications in Super Resolution Microscopy
Session Chair: Dr Jessica Valli (Heriot-Watt University, UK)
The emergence of super resolution light microscopy techniques in the last quarter-century has allowed researchers to probe further into the hitherto unseen depths of biology and ‘resolve’ many previously-unanswered questions. While there are now several commercially-available super resolution systems, these techniques are still anything but mundane, and new developments continue to push the limits of resolution closer to those achievable by electron microscopy. This session will focus on both the most recent developments in super resolution microscopy and the applications of these techniques to biological frontiers.
Professor Dr Markus Sauer (University of Würzburg, Germany)
Talk Title: Present, future and past of super-resolution microscopy by dSTORM
Markus Sauer studied Chemistry at the University of Heidelberg where he received his Diploma in 1991 and finished his PhD 1995 in Physical Chemistry. 1998 he has been awarded the BioFuture Prize for Detection, Analysis and Handling of Single Molecules, which allowed him to establish his own group for single-molecule fluorescence detection and single-molecule DNA sequencing. Since 2009 he is Professor and Chair of the Department of Biotechnology and Biophysics at the Julius Maximilian University Würzburg. His research interests are single-molecule fluorescence spectroscopy and imaging with a particular focus on super-resolution fluorescence imaging by direct stochastic optical reconstruction microscopy (dSTORM) and its applications in structural biology, immunology, host-pathogen interactions, and cancer immunotherapies. A couple of years ago he started to combine refined super-resolution and expansion microscopy methods to push the limits and pave the way for true molecular resolution. He has published more than 280 journal papers and coordinates several super-resolution microscopy based projects.
- Talk Title: Present, future and past of super-resolution microscopy by dSTORM
Dr Katrin Willig (Max Planck Institute, Germany)
Talk title: Long-term and multi-colour STED microscopy of the living mouse brain
Katrin studied Physics at Würzburg University, Germany, and at the University of New Mexico, Albuquerque, U.S.A., before joining Stefan W. Hell’s research team at the Max Planck Institute for Biophysical Chemistry in Göttingen in 2002 for her PhD on STED microscopy. In this time she pioneered the first biological relevant applications of super-resolution microscopy and the use of fluorescent proteins for STED microscopy of living cells. During her postdoc she developed STED microscopy for imaging tissue, i.e. living organotypic brain slices and demonstrated the strength of these technologies by in vivo imaging of the tiny protrusions (dendritic spines) on nerve cell dendrites inside a living mouse brain with unprecedented detail. Since 2014 she is heading her own junior research group at the Göttingen CNMPB located the Max Planck Institute of Experimental Medicine. Her objective is to further the strength of STED microscopy for in vivo applications. In 2015 she was awarded the Lennart Nilsson Award “Recognizing Extraordinary Image Makers in Science” from Karolinska Institutet.
- Talk title: Long-term and multi-colour STED microscopy of the living mouse brain
Frontiers in Bioimaging: Lightsheet Microscopy: Development and Application
Session Chair: Dr Mike MacDonald (University of Dundee, UK)
Lightsheet microscopy has been with us for over a century, but it is the recent advances in camera technology and computational power that have allowed it to flourish. Initially driven largely by the demand for live, widefield, volumetric imaging in developmental biology, lightsheet imaging is now a tool which is used in a wide variety of applications. In fact, it has even been recently used for histopathological studies of COVID-19. This more established status has not stopped lighsheet imaging from evolving technologically and this session will focus on, and welcome submissions covering, the development through to the application of lightsheet imaging, and especially those doing both.
Dr Caren Norden (Instituto Gulbenkian de Ciência, Portugal)
Talk title: Making the retina: from cells to tissue and back again
Caren Norden is a cell and developmental biologist who is the head of the Cell Biology of Tissue Morphogenesis Research Group at the IGC in Oeiras Portugal. Her research aims to understand ‘how cells form tissues’ using the vertebrate retina as a model system and investigating phenomena across scales and using cross-disciplinary approaches.
She studied in Hannover, Germany and did her masters at the the Mount Sinai school of Medicine in New York City studying the emergence of polarity of neurons in culture. In 2002 she started studying polarity and cytokinesis in the budding yeast saccharomyces cerevisiae. she used her knowledge in cell biology and moved to understand neuroepithelial development in zebrafish at the University of Cambridge, UK during her postdoc. In 2010, she started her own group at the MPI-CBG in Dresden, where she expanded her research portfolio and made important discoveries in different contexts of retinal development.
Caren Norden is the deputy director of science of the Instituto Gulbenkian de Ciência since October 2019. In 2002 she started her PhD At the ETH Zurich in Switzerland. Her works has previously been recognized by an HFSP Career Development Award and the José A. Campos-Ortega Young Scientist Award of the German Society for Developmental Biology. In addition, she became a member of the EMBO Young Investigator Network in 2015 and full EMBO member since 2020.
- Talk title: Making the retina: from cells to tissue and back again
Professor Dr Alexander Rohrbach (IMTEK, University of Freiburg, Germany)
Talk Title: Novel approaches to improve image quality in light-sheet microscopy
I studied physics at the University of Erlangen-Nürnberg (Germany) with diploma at the institute of optics in 1994. During my PhD in physics in Heidelberg I investigated different kinds of light scattering at the Kirchhoff-Institute for Physics, as well as evanescent wave microscopy at the Max-Planck-Institute for medical research in Heidelberg – both with applications in cell biology. After my PhD in 1998, I continued my research as a Post-Doc at the European Molecular Biology Laboratory (EMBL) in Heidelberg. I intensified my studies on microscopy, light scattering and optical forces. In 2001, I became project leader at EMBL, where I concentrated on the development of photonic force microscopy and on applications in biophysics and soft matter physics. In 2005, I was awarded with the habilitation in physics at the university of Heidelberg. Since January 2006 I am full professor for Bio- and Nano-Photonics at IMTEK, Faculty of engineering and since 2007 also a member of the physics faculty, University of Freiburg. We want to understand the dynamics of living system on short timescales. Therefore, we develop novel techniques for laser optical microscopy and optical force based applications. We investigate the biophysics of living cells and of bio-mimetic systems based on their nano-mechanics and thermal fluctuations. Since 1999 I have been fascinated by microscopy with more than one optical axis. Being in Freiburg, I introduced Bessel beams to the field of light-sheet microscopy in 2009 and 2010, the concept of line-confocal detection in 2012, and a powerful method to suppress scattered light image contributions in 2016.
- Talk Title: Novel approaches to improve image quality in light-sheet microscopy
Frontiers in Bioimaging: Correlative imaging of Organelle Organization and Architecture
Session Chair: Dr Sebastian Munck (VIB Bio Imaging Core KU Leuven, Belgium)
In this session, we bring together the latest news on correlative imaging. We will be exploring the interface of cell and structural biology by combining the power of different approaches. Talks will be highlighting different approaches to study cellular organization, including the architecture of the membranes, compartmentalization, and how organelle architecture directs molecular function. In addition, we will emphasize the correlative aspects of the techniques employed and how to combine them efficiently. Among the methods, we will be discussing is cryo-electron tomography to visualize macromolecules directly and in situ and how to leverage this information in combination with, for example, the selectivity of fluorescence approaches to analyze molecular organization within the cell at the scale of single molecules.
Dr Kristina Micheva (Stanford University, USA)
Talk title: Correlative imaging of the brain with array tomography
Kristina D. Micheva is Senior Research Scientist in the Department of Molecular and Cellular Physiology at Stanford University, California, USA. She obtained her Ph.D. with Dr. Clermont Beaulieu at Université de Montréal in Canada and later trained as postdoctoral fellow with Dr. Peter S. McPherson at McGill University in Montreal, Canada and Dr. Stephen J Smith at Stanford University. She is coinventor of array tomography, a high-resolution proteomic imaging method that allows the imaging of dozens of different antibodies at individual subcellular structures within large volumes of tissue. Dr. Micheva is interested in synapse organization, function and plasticity, and uses correlative array tomography to explore the diversity of synapses, axons and their myelin in experimental animals and human patients.
- Talk title: Correlative imaging of the brain with array tomography
Dr Benjamin Engel (Helmholtz Zentrum München, Germany)
Talk title: Exploring Molecular Landscapes Inside Cells with In Situ Cryo-Electron Tomography
Benjamin Engel received his bachelor’s degree from UC Berkeley and his Ph.D. from UC San Francisco. He performed his postdoctoral studies in the lab of Wolfgang Baumeister at the Max Planck Institute of Biochemistry. He currently leads a group at the Helmholtz Pioneer Campus in Munich, Germany, where he studies the molecular architecture of organelles.
Cells accomplish the biochemical reactions of life by concentrating their proteins into a variety of subcellular compartments called organelles. Our group explores the relationship between the form of the organelle and the function of its resident macromolecules. How does organelle architecture direct molecular function, and reciprocally, how do macromolecules sculpt and shape organelles? To investigate these questions, we use focused ion beam (FIB) milling of frozen cells followed by cryo-electron tomography (cryo-ET) to image macromolecules within their native cellular environment. Through a combination of nanometer-precision localization and high-resolution structural analysis, we aim to chart the molecular landscapes of organelles. Learn more about us here: https://www.cellarchlab.com/
- Talk title: Exploring Molecular Landscapes Inside Cells with In Situ Cryo-Electron Tomography
Frontiers in Bioimaging: Quantifying Dynamic Movement in Living Cells
Session Chair: Dr Steve Briddon (University of Nottingham, UK)
The precise co-ordination of molecular interactions in cell compartments fundamentally defines cell behaviour. These movements and interactions between proteins, peptides, lipids and DNA in live cells can be quantified and described using a range of advanced imaging methods, such as Resonance Energy Transfer (RET) techniques (FRET/FLIM), fluorescence fluctuation approaches (FCS etc.) and single particle tracking (SPT). The huge expansion in the availability of these techniques have transformed quantitative biology and allowed robust means to characterise spatiotemporal changes in molecular interactions. This session welcomes studies using advanced or novel techniques in this area, particularly those targeted to living cells.
Professor Philipp Kukura (University of Oxford, UK)
Talk title: Mass photometry: weighing molecules with light
Philipp Kukura is a Professor of Chemistry at the University of Oxford, leading an interdisciplinary research group that focusses on the development and application of new optical methodologies to study biomolecular structure, dynamics and interactions. A particular focus has been mass photometry, the detection, characterisation and quantification of biomolecular structure and interactions by mass measurement at the single molecule level in solution. Recent awards include those by the RSC (Harrison-Meldola 2011 and Marlow 2015), the European Biophysical Society Association (Young Investigator Medal 2017), a Royal Society Wolfson Research Merit Award (2018), the Klung-Wilhemy Award (2018) and a UK Blavatnik Award Laureate (2019).
Professor Philipp Kukura is the winner of the 2021 RMS Medal for Light Microscopy, this will be presented at mmc2021.
- Talk title: Mass photometry: weighing molecules with light
Dr Vladana Vukojevic (Karolinska Institute, Sweden)
Talk title: Quantitative Scanning-Free Confocal Fluorescence Microscopy for the Characterization of Fast Dynamic Processes in Live Cells
Dr. Vladana Vukojević is an Associate Professor of Biochemistry at Karolinska Institutet, Stockholm, Sweden. Her research focuses on understanding how biomolecules are integrated via chemical reactions and transport processes into self-regulated dynamical networks of interactions through which life-sustaining biological functions emerge at longer spatio-temporal scales. To this aim, she is using analytical and integrative approaches. Her experimental work focuses on further advancement of existing and the development of new fluorescence microscopy and correlation spectroscopy based methodologies, such as the recently developed quantitative time-resolved scanning-free confocal fluorescence microscopy via massively parallel Fluorescence Correlation Spectroscopy. She is using these advanced analytical methods to map the dynamic reaction-diffusion landscape in live cells and to quantitatively characterize how it is altered by selected substances, e.g. prospective drug candidates or disease causing agents. Moreover, she is using approaches from dynamical systems theory to describe and predict dynamic changes in complex biochemical systems and interpret their consequences. Dr. Vukojević is Visiting Professor at the University of Belgrade, Serbia; Visiting Lecturer at the Hokkaido Summer Institute (HSI), Hokkaido University, Sapporo, Japan; and co-founder of AmyloiDia, Sweden AB. She is co-author of more than 90 research articles in renowned peer-reviewed scientific journals.
- Talk title: Quantitative Scanning-Free Confocal Fluorescence Microscopy for the Characterization of Fast Dynamic Processes in Live Cells
Imaging in Development and Disease
Session Chair: Dr Brian Stramer (King’s College London, UK)
Our ability to image cells in fixed and living tissues is transforming our view of embryogenesis and disease progression. This session aims to highlight the latest developments of high-resolution tissue imaging, which examines either live or fixed samples across a range of spatial and temporal scales. Contributions to this session are encouraged from any area of research where imaging techniques are being applied to the study of animal development and normal or pathological physiology.
Dr Yanlan Mao (University College London, UK)
Talk title: Coping with mechanical stress: Tissue dynamics in growth and repair
Yanlan Mao is a Group Leader at the MRC Laboratory for Molecular Cell Biology, University College London. After receiving her BA in Natural Sciences at Cambridge University, she completed her PhD with Matthew Freeman at the MRC LMB in Cambridge on Drosophila cell signaling and epithelial patterning. During her postdoc with Nic Tapon at the CRUK London Research Institute (now Francis Crick Institute), she became interested in tissue mechanics and computational modeling approaches, and studied the role of mechanical forces in orienting cell divisions and controlling tissue shape. In 2014, when Yanlan started her independent research group, she has continued to investigate the role of mechanical forces in tissue development, homeostasis and repair.
She now holds a MRC Career Development Award Fellowship, a Lister Institute Prize and an L’Oreal UNESCO Women in Science Fellowship. She was selected to join the EMBO Young Investigator Programme in 2018, was recently awarded the Early Career Prize in Mechanobiology by the Biophysical Society, and the BSCB Women in Cell Biology Early Career Medal.
Dr Yanlan Mao is the winner of the 2021 RMS Medal for Life Sciences, this will be presented at mmc2021.
- Talk title: Coping with mechanical stress: Tissue dynamics in growth and repair
Dr Colinda Scheele (VIB-KU Leuven Center for Cancer Biology, Belgium)
Talk Title: Unmasking in vivo stem cell dynamics in mammary tissue and mammary tumours
Colinda Scheele is a junior group leader at VIB-KU Leuven Center for Cancer Biology as of June 2020. Colinda received a Master in Biomedical Sciences from Utrecht University. She performed her PhD research (cum laude) in the lab of Prof. Jacco van Rheenen (at the Hubrecht Institute (Utrecht) and the Netherlands Cancer Institute (Amsterdam)). During this time, she pioneered unbiased lineage tracing approaches, 3D whole organ imaging techniques and intravital microscopy strategies to study mammary stem cell dynamics. She identified the pubertal mammary stem cells and how they drive mammary gland branching morphogenesis (Scheele*, Hannezo* et al., 2017, Nature; Hannezo*, Scheele* et al., 2017, Cell; Corominas-Murtra*, Scheele* et al., 2020, PNAS). Moreover, she studied how these mammary stem cells contribute to tumor initiation and progression. For this research proposal, Colinda was awarded a prestigious Boehringer Ingelheim Fonds (BIF) PhD fellowship.
The Scheele laboratory focuses on morphogenesis, homeostasis, and tumorigenesis of epithelial organs ranging from a cellular level to the organ scale. More specifically, the Scheele lab studies how healthy tissue architecture and environment prevent or promote the different steps of tumorigenesis. To study these highly dynamic processes, the Scheele lab utilizes and develops state-of-the-art imaging approaches, including 3D whole organ imaging and 4D intravital microscopy using imaging windows. These imaging tools are complemented with (spatial) omics approaches, as well as quantitative modelling to further elucidate the mechanisms of tissue transformation.
- Talk Title: Unmasking in vivo stem cell dynamics in mammary tissue and mammary tumours
Up Close with the Enemy: Imaging Pathogen-host Dynamics
Session Chair: Dr Leandro Lemgruber (University of Glasgow, UK)
The continuous technology development in both light and electron microscopy and the use of correlative microscopy (combining different types of microscopy) have provided exciting opportunities to address novel and important questions relating to the cellular structure of human pathogens and their relationship with the host, both in vitro and in vivo. This session aims to bring the recent works in pathogen imaging and analysis and show how advanced microscopic techniques are continuing to provide far-reaching insights into the biology of pathogens.
Dr Maximiliano Gutierrez (The Francis Crick Institute, UK)
Talk title: Mycobacterium tuberculosis interactions with host macrophages in space and time
Max is a cell biologist originally from Mendoza, Argentina. In 2005, he obtained a PhD in cell biology from the University of San Luis, Argentina. During his PhD work, he discovered a novel innate immune pathway, later named “Xenophagy”. In 2006, he moved to EMBL in Heidelberg, Germany as a postdoc in Gareth Griffiths Laboratory, first as a fellow of the Alexander von Humboldt Foundation and then as an EMBO fellow. His work in Heidelberg focused on the cell biology and imaging of macrophages; it was also in Heidelberg that he felt in love with Electron Microscopy.
In 2009, he started his independent research group at the Helmholtz Centre for Infection Research in Braunschweig, Germany as head of the Junior Research Group 'Phagosome Biology'. In 2012, he was recruited as a Programme Leader Track at the Medical Research Council's National Institute for Medical Research, which became part of the Francis Crick Institute in 2015. Since 2018, he is a Senior Group Leader at the Francis Crick Institute.
As a cell biologist trained in microbiology, how intracellular pathogens evolved strategies to survive within host cells has always fascinated me and clearly shaped my scientific career. My long-standing interest is the cellular mechanisms that regulate the interactions between Mycobacterium tuberculosis and host cells. I aim to better understand the host cell factors that contribute to M. tuberculosis control as well as the M. tuberculosis factors that this pathogen uses to highjack host cells. To this end, my lab is developing a variety of cutting-edge imaging technologies in high containment combined with various model systems and approaches at the single cell level.
- Talk title: Mycobacterium tuberculosis interactions with host macrophages in space and time
Professor Torsten Ochsenreiter (University of Bern, Switzerland)
Torsten did his PhD at the University of Darmstadt in Germany, in microbial ecology using metagenomic approaches to characterise the impact of archaea mostly in soil habitats. He then switched gears and fell in love with parasites. During his postdoc first at the MBL in Woods Hole, USA and then at UGA in Athens, GA USA, he worked on RNA-editing in the human and animal parasite Trypanosoma brucei. After moving to the University of Bern in Switzerland he focused on cell biological aspects of mitochondrial organelle biology in trypanosomes using a variety of techniques most importantly super resolution microscopy. His lab continues to apply high end microscopy techniques to visualise parasite cell biology including ultrastructure expansion microscopy and cryo-electron microscopy.
- Talk title:
Seeing is Believing – Multiplexed Imaging Flow Cytometry
Session Chair: Dr Ziv Porat (Weizmann Institute of Science, Israel)
Imaging Flow Cytometry (IFC) combines the information-rich imagery of microscopy with the high-throughput, statistically robust strength of Flow Cytometry. IFC can simultaneously image up to 10 fluorescent channels as well as bright field and dark field images, at a rate of up to 5000 cells/sec. In addition to rapid, uniform high-resolution and multi-parametric acquisition, IFC has special advantages in identification of rare populations and hard-to image cells. This session will showcase state-of the art, novel applications demonstrating the unique abilities and advantages of IFC across a broad base of biological fields.
Dr Orla Maguire (Roswell Park Cancer Institute, USA)
Talk title: Monitoring immune responses by imaging flow cytometry
Dr. Maguire is a Senior Cytometry Specialist in the department of Flow and Imaging Cytometry at Roswell Park Cancer Institute, Buffalo, NY. She obtained her PhD from Ulster University in Northern Ireland, UK, studying the activity of nuclear receptor transcription factor pathways in cancer. Her current research interests are focused on developing clinical applications of imaging flow cytometry (IFC), with special attention to transcription factor pathways. The goal of this research is to provide clinicians and researchers with useful information in the functional activity of transcription factor pathways in immunophenotypically defined populations. More recently her research has extended to cellular communication via exosome signaling using IFC. She has published numerous original method manuscripts in these areas and currently has national and international collaborations with both hospital-based research groups and pharmaceutical companies studying signaling pathways in several diseases. Dr. Maguire is an ISAC Marylou Ingram Scholar for the 2018-2022 term.
- Talk title: Monitoring immune responses by imaging flow cytometry
Dr Yoav Shechtman (Israel Institute of Technology, Israel)
Talk title: Nanoscale 3D co-Localization in Imaging Flow Cytometry via Point-Spread-Function Engineering
Assistant Professor Yoav Shechtman leads the Nano-Bio-Optics lab at the Technion, Israel Institute of Technology. Yoav Finished all degrees at the Technion: BSc in Physics and Electrical Engineering (2007), Phd (2013), and then spent three years as a postdoc at Stanford University (2016), developing super-resolution microscopy methods with W.E. Moerner.
His research interests lie mainly in developing and applying optical and signal processing methods for nanoscale imaging challenges. Specifically, Yoav focuses on aspects of single/multiple particle localization and tracking under challenging conditions, e.g. three-dimensional, multicolor, and high throughput imaging, using advanced optical, signal processing and machine learning techniques. The techniques developed in the lab are applied to challenges ranging from basic science, i.e. observing chromatin dynamics in 3D, to biomedical applications, i.e. developing ultra-sensitive assays for bacterial growth.
Among Yoav's recent awards and recognitions: 2016 Technion Career Advancement Chair, 2017 Zuckerman Faculty Scholar, 2018 Early Career Award of the International Association for Medical and Biological Engineering (IAMBE), 2018 European Research Council starting grant, 2019 Uzi and Michal Halevy Award for Innovative Applied Engineering, 2020 International Union for Pure and Applied Biophysics (IUPAB) Young Investigator Prize and Medal.
Lab wesbite: http://nanobiooptics.net.technion.ac.il/
- Talk title: Nanoscale 3D co-Localization in Imaging Flow Cytometry via Point-Spread-Function Engineering
Session Chair: Dr Karen Hogg (University of York, UK)
High-plex imaging cytometry enables the scrutiny of cells using a high number of quantitative characteristics whilst maintaining their spatial locations. Probes specific for molecular and protein targets provide information of cellular interactions in complex biological systems such as cancer, immunology, cell development, infection and disease. Recent advances in imaging technologies have led to the deep profiling of the tissue microenvironment, the identification of new biomarkers and previously unknown cellular interactions that would have been previously lost in dissociated tissue. Speakers shall provide an expert insight into this application-rich form of imaging and how it has been of benefit to their research.
Dr Nidhi Sharma Dey (University of York, UK)
Talk title: Immunopathology pipeline to study potential host directed therapy targets in cutaneous leishmaniasis
Nidhi is a Postdoctoral Research Associate in the Kaye lab, Hull York Medical School, University of York. Trained in developmental genetics (PhD), Nidhi completed her PhD (2017) from Indian Institute of Science, Education and Research (IISER), India looking for stem cells in Drosophila as a model for aorta-gonad-mesonephros–derived hematopoietic stem cells . Currently, Nidhi is investigating immunopathology of human CL with particular emphasis on host -immune interactions and looking for novel host directed therapies that might alleviate patient suffering.
- Talk title: Immunopathology pipeline to study potential host directed therapy targets in cutaneous leishmaniasis
Dr Jan Roger (GlaxoSmithKline, UK)
Talk title: Application of tissue-based spatial multiplex imaging technologies in drug discovery
Jan studied Biochemistry at University of Warwick, before gaining a PhD at University of Nottingham using Molecular Histology to investigate the expression and turnover of extracellular matrix proteins.
He has been working in Pharma molecular histology groups since 2007, leading tissue based assay development and deployment on drug discovery programmes in respiratory, oncology and immunology therapy areas. This has included all stages of the drug discovery pipeline, from broad target identification campaigns, through drug efficacy and tox studies, and regulated clinical trial assay delivery. This has included in depth experience with brightfield and fluorescence IHC and ISH, image analysis, and antibody and assay validation.More recently Jan has built a team dedicated to the application of spatial multiplexing and multi-omics, combining diverse skill sets from imaging, proteomics, sequencing and data analytics, to maximise the impact of tissue slide-based multiplexing technologies in applications such as immuno-phenotyping tissues, and characterising molecular pathways driving mechanism and mode of action at the cellular level in tissue context.
- Talk title: Application of tissue-based spatial multiplex imaging technologies in drug discovery
Volume Microscopy in Physical and Life Sciences
Session Chair: Xiangli Zhong (University of Manchester, UK) & Dr Saskia Lippens (VIB Ghent, Belgium)
Volumetric microscopy has become one of the most prominent subjects in both the Physical, as well as the Life Sciences, enabled by the rapid development of innovative tools for 3D imaging and spectroscopy such as analytical focused ion beam (FIB) systems for serial sectioning. Three dimensional visualisation significantly enhances our understanding from the macroscopic to the sub-nanometer level in a wide range of systems from metals via ceramics to biocomposites, biominerals and soft biological tissues. The close interaction between Physical and Life Sciences has been an important driver for this progress irrespective of length scale or resolution of the technique. Besides FIB-based methods, relevant examples of emerging techniques include serial-section SEM and TEM, electron Tomography, X-ray micro- and nano-CT, array tomography and gridTAPE TEM . This session containing both contributed and invited talks and posters discusses recent advances in volumetric characterisation techniques using ion beams, X-rays and electrons and focuses on recent novel applications. This session is linked to a separate specific FIB & Sample Preparation workshop which discusses approaches and problems associated with FIB applications and sample preparation methods which enable volume microscopy.
Professor Roland Kröger (University of York, UK)
Talk title: Three-dimensional analysis of hierarchical biocomposites using electron microscopy: Preparation and structure evaluation across the length scales
Roland is a Professor at the Department of Physics at the University of York concentrating on Nano- and Biomaterials using electron microscopy as well as various spectroscopy tools including Raman microscopy and X-ray techniques. He obtained his PhD from the University of Hamburg/Germany and the Fraunhofer Institute for Surface Science and Technology in Braunschweig/Germany. Roland has since built a large expertise in Materials Physics and Materials Science covering diamond thin films, metal/semiconductor nanostructures, nitride based light-emitting devices, metal nanoparticles for biomedical applications and biominerals using focused ion beam as a key method for sample preparation and analysis. Besides his interest in multi-lengthscale material characterization in 3D he is particularly focussing on in situ techniques to study mineralisation processes in liquid environments.
- Talk title: Three-dimensional analysis of hierarchical biocomposites using electron microscopy: Preparation and structure evaluation across the length scales
Professor Philip Withers (Henry Royce Institute , University of Manchester, UK)
Talk title: Large volume three dimensional correlative microcopy
Philip Withers is the first Regius Professor of Materials and Chief Scientist of the Henry Royce Institute for Advanced Materials. He was a lecturer in Cambridge from his PhD until 1998 before taking up a Chair in Manchester. He employs advanced imaging and characterisation techniques to follow the behaviour of engineering and natural materials, often in 3D and in operando. A fellow of the Royal Society and the Royal Academy of Engineering. He pioneered the use of neutron and synchrotron X-rays for probing the behaviour and degradation of engineering materials. In 2008 he set up the Henry Moseley X-ray Imaging Facility, one of the most extensive suites of X-ray Imaging facilities in the world with a special focus on in situ time lapse 3D X-ray imaging. In 2014, the Facility was awarded the Queen’s Anniversary Prize. In 2020 these facilities became a founding part of the National Research Facility in Lab. CT opening up the capability to the wider academic and industrial community. He is also active in the area of 3D electron microscopy imaging and characterisation through dual beam (PFIB-SEM) and tribeam (laser-PFIB-SEM) serial sectioning. Taken together the X-ray and electron microscopy techniques cover a very wide range of length scales and contrast mechanisms and these are being incorporated within a correlative framework to provide multifaceted, multiscale information.
- Talk title: Large volume three dimensional correlative microcopy
X-ray Microscopy: A Powerful Tool to aid the Understanding of Structures in Life and Physical Sciences
Session Chair: Dr Liz Duke (EMBL Hamburg, Germany)
Thanks to Roentgen’s work in 1895 the use of X-rays as a research tool in the life and physical sciences is not new. However in recent years their use has increased, particularly but not exclusively in the area of X-ray imaging, aided by developments in many areas including sample preparation, detector technology and availability of commercial laboratory based microscopes. In this session we aim to explore the use of X-ray microscopy in life and physical sciences. We look forward to hearing from those who have used X-rays in their work either as a single technique or in conjunction with other microscopy techniques. The session will be bookended by invited talks. A further three talks will be selected from submitted abstracts and three posters will be chosen for flash talks.
Dr Julia Parker (Diamond LIght Source, UK)
Talk title: Using Multimodal X-ray Microscopy for Structural and Chemical Nanoscale Imaging
Julia Parker is an X-ray microscopist at Diamond Light Source, the UK’s national synchrotron facility. Julia is responsible for the operation of the hard X-ray nanoprobe beamline, supporting nanoscale spectroscopy, diffraction and imaging experiments across the life and physical sciences. Julia joined Diamond in 2007 after completing her PhD at the University of Cambridge. Julia’s current research interests lie in the area of biomineralisation, using synchrotron techniques to unveil details of the structure of calcium carbonates formed by organisms such as shells and studying the crystallisation and formation pathways of calcium carbonates.
- Talk title: Using Multimodal X-ray Microscopy for Structural and Chemical Nanoscale Imaging
Dr Alexandra Pacureanu (ESRF, The European Synchrotron, France)
Talk title: X-ray microscopy for deep and precise bioimaging
Alexandra Pacureanu is a scientist at the European Synchrotron developing X-ray microscopy with focus on bioimaging. She has a background in electrical engineering and signal processing, and she received her PhD from INSA Lyon, France in 2012. Her thesis on nanoscale X-ray tomography of the bone cell network received the French Biomedical Engineering Society innovation award. Between 2012 and 2014 she was a postdoctoral fellow at Uppsala University and the Science for Life Laboratory in Sweden, where she developed image analysis methods for in-situ gene sequencing and high throughput microscopy. For the following five years she returned to ESRF to work on development of X-ray cryogenic nano-tomography for 3D imaging of biological tissues and cells. In 2019 she was a visiting scientist in the Neurobiology department at Harvard Medical School in Boston, and a senior research fellow at the University College London and the Francis Crick Institute in the Sensory Circuits and Neurotechnology group. Since the beginning of 2020 she is leading the X-ray Neuroimaging at Nanoscale research unit at ESRF.
Dr Alexandra Pacureanu is the winner of the 2021 Alan Agar Award for Electron Microscopy Medal, sponsored by Agar Scientific. This will be presented at mmc2021.
- Talk title: X-ray microscopy for deep and precise bioimaging
Machine Learning for Image Analysis
Session Chair: Dr Martin Jones (The Francis Crick Institute, UK)
Artificial intelligence based methods, such as machine learning and deep learning, have revolutionised many aspects of image analysis in a broad range of subject areas. As the different communities get to grips with the technical implementation of these methods, there has been a shift in focus to getting cutting-edge tools into the hands of domain experts, who are often not computational experts, with improvements in workflows and accessibility. This session will focus on state-of-the-art AI based image analysis methods and efforts to ensure these tools are made accessible to the wider communities in both life and physical sciences.
Dr Kwasi Kwakwa (European Molecular Biology Laboratory -EBI, UK)
Talk title: The integration of machine learning tools into spatial transcriptomics image acquisition and analysis
Kwasi Kwakwa is an Imaging Scientist at the European Molecular Biology Laboratory-EBI working jointly between the Marioni group at the EBI and the Bayraktar group at the Wellcome Sanger Institute. He obtained his PhD from King’s College London in 2013 and has been primarily involved in microscope design, single molecule imaging and image analysis ever since. Currently he is part of a group interested in building imaging and image analysis tools for spatial transcriptomics.
- Talk title: The integration of machine learning tools into spatial transcriptomics image acquisition and analysis
Deborah Schmidt (MPI of Molecular Cell Biology and Genetics, Germany)
Talk title: User friendly integration of Deep Learning into scientific applications
Deborah Schmidt recently joined the Image Data Analysis group led by Kyle Harrington at the Max Delbrück Center for Molecular Medicine in Berlin. Since 2017 she integrated Deep Learning solutions into Fiji and became a core contributor to the SciJava / ImageJ2 ecosystem as a member of the Jug Lab at the Center for Systems Biology / Max-Planck Institute for Molecular Cell Biology and Genetics in Dresden. Occasionally she creates generative artwork and interactive installations and is fascinated by frugal science, open source communities, and decentralized systems.
- Talk title: User friendly integration of Deep Learning into scientific applications
Chemical Imaging of Biological Samples using Electron, Ion and X-ray based Techniques
Session Chair: Dr Errin Johnson (University of Oxford, UK)
Microanalysis methods, such as Energy Dispersive X-ray spectrometry (EDS), Electron Energy Loss Spectroscopy (EELS) and Nano-Secondary Ions Mass Spectroscopy (NanoSIMs) are more traditionally associated with physical science than biological applications. However, this is starting to change, with these techniques and others, such as Synchrotron Radiation X-ray Fluorescence (SRXRF), increasingly being used to link chemical composition with ultrastructure in biological samples. For instance, EDS has recently been used to identify secretory cells in pancreatic islets by the elemental makeup of their granules, while NanoSIMs can pinpoint the cellular locations of isotope-labelled metabolites, hormones, drugs and lipids. This interdisciplinary session will include talks from physical scientists and biologists who are applying these techniques to a variety of biological and biomedical samples. It will cover biological sample preparation requirements for chemical imaging, potential challenges, opportunities for multimodal imaging and application examples.
Dr Ben Giepmans (University Medical Center Groningen, Netherlands)
Talk Title: Large-scale multimodal correlated microscopy in biomedicine
Ben Giepmans is intrigued by how molecules act together to control cell fate in health and disease. He is a cell biologist and molecular biochemist/ microscopist and always have been triggered by using newly developed techniques to overcome current limitations in research, especially when these techniques allow to get unbiased, unexpected answers to research questions. His PhD research at The Netherlands Cancer Institute and related work in The Scripps Research Institute have led to a better molecular understanding of regulation of gap junctions. Similarly, during his post-doc at the National Center of Microscopy and Imaging Research (University of California, San Diego), we optimized and implemented several new advanced imaging techniques and probes to study protein dynamics in live cells and protein localization at high resolution. Giepmans was awarded the Robert Fuelgen prize by the Society for Histochemistry for this work. Currently, my team develops and/or implements new imaging techniques and probes for microscopy, including large-scale EM (‘nanotomy’) and ‘ColorEM’, where elemental analysis is implemented in EM for life sciences. Next to his research, Giepmans took the challenge to build an advanced microscopy imaging infrastructure at the UMCG. The center is now equipped with state-of-the-art light microscopes and electron microscopes (www.umic.info). We continue to develop and/or implement multimodal microscopy in several research projects (www.nanotomy.org). In particular, we study the possible triggers that may interfere with normal function of Islets of Langerhans ultimately resulting in Type 1 diabetes.
- Talk Title: Large-scale multimodal correlated microscopy in biomedicine
Dr Katie Moore (University of Manchester, UK)
Talk title: Applications of high spatial resolution SIMS imaging (NanoSIMS) in plant science
Katie Moore is a Lecturer in Materials Characterisation in the Department of Materials at the University of Manchester. She obtained her D.Phil from the University of Oxford in Materials Science in 2011 and then held an EPSRC Postdoctoral Research Fellowship working on NanoSIMS imaging of trace elements in crops before taking up a Research Fellow position at the University of Manchester in 2014. Katie has been working with the NanoSIMS since 2008 and now leads the NanoSIMS group at Manchester University.
Her research has been primarily to use the NanoSIMS to investigate trace element uptake in crops, such as arsenic uptake in rice and iron in wheat grain, but she has also used the NanoSIMS on a wide range of other materials. Recent work includes investigating oxidation and hydrogen pick up mechanisms in zirconium, steel and nickel alloys using isotopic tracers, such as deuterium, with the NanoSIMS.
- Talk title: Applications of high spatial resolution SIMS imaging (NanoSIMS) in plant science
Label Free Imaging
Session Chair: Dr Natalie Belsey (National Physical Laboratory, UK)
Label-free approaches have provided valuable new tools for structural and chemical visualisation of systems where labelling is not possible or desirable, for example medical imaging and other in situ applications. These powerful techniques have also enabled the study of systems where the presence of a tag would cause excessive perturbation to the sample, for example small molecule pharmacokinetics. This session welcomes a broad range of label-free optical microscopy techniques, such as vibrational spectroscopic imaging, multiphoton imaging, Brillouin scattering, interference and phase-contrast microscopies and holotomography. Particular consideration will be given to the elucidation of quantitative information using these methods.
Professor Minbiao Ji (Fudan University, China)
Talk title: Deep-learning based stimulated Raman scattering histology
Dr. Minbiao Ji received B.S. degree in Physics from the Peking University, Beijing, in 2005 He received his Ph.D degree in Physics at Stanford University, in 2011. His research was focused on using ultrafast laser spectroscopy. Afterwards, he joined the research group of Prof. Sunney Xie at Harvard University as a postdoctoral research fellow, working on coherent Raman scattering microscopy. Minbiao Ji is currently a Professor in the Department of Physics at Fudan University in Shanghai. His current research is focused on developing novel nonlinear optical spectroscopy and microscopy tools to study biomedical problems and material sciences.
- Talk title: Deep-learning based stimulated Raman scattering histology
Professor Malgorzata Kujawinska (Warsaw University of Technology, Poland)
Talk Title: Optical diffraction tomography: present solutions and future trends in biomedical applications
Malgorzata Kujawinska PhD DSc., SPIE Fellow, Full Professor of applied optics at Warsaw University of Technology. Expert in full-field optical metrology, development of novel photonics measurement systems, 3D label-free, quantitative imaging in biomedical engineering with special focus on digital holographic microscopy and optical diffraction tomography. Author of one monograph, several book chapters and more than 250 papers in international scientific journals. She had been the SPIE President and vice-President of European Technology Platform Photonics21. The recipient of SPIE 2013 Chandra S. Vikram Award in Optical Metrology.
- Talk Title: Optical diffraction tomography: present solutions and future trends in biomedical applications
3D+ Image Analysis
Session Chair: Dr Chas Nelson (University of Glasgow, UK)
Imaging and microscopy in both the life and physical sciences spans dimensions including 3D imaging or the inclusion of temporal or spectral information and often all three. Analysing data in these higher dimensions comes with it’s own challenges and often requires bespoke mathematical and algorithmic solutions. This session will focus on state-of-the-art image analysis that enables scientists from both life and physical sciences to answer scientific questions in a quantitative fashion from multidimensional imaging.
Dr Perrine Paul-Gilloteaux (Université de Nantes, France)
Talk title: Correlative microscopies: quantitatively fusing information from 3D+ to 6D+
Perrine Paul-Gilloteaux is a CNRS research engineer in image data analysis and management, in Nantes. After electrical engineering studies at the national institute of applied science of Rennes, she received her PhD in 2006 from the University of Rennes, France, in biomedical engineering. After a post-doctoral position in Maynooth, Ireland, in systems biology, she was recruited by CNRS at the institut Curie in Paris in 2010 as bio image analyst. She moved to Nantes, France in 2015, where she is now heading the MicroPICell facility (light microscopy, histology and image analysis), part of the France Bio Imaging national infrastructure, and center of excellence Nikon. In addition to developing image analysis methods as services for the facility users, she is the PI for the development of methods for correlative microscopies, implemented in ec-clem software. Her main research interests are the development of methods for quantitative analysis of dynamics in cell biology and for the fusion and interpretation of multimodal and multiscale data. She is one of the founder and working group leader in the COST action network NEUBIAS, network of european bio image analyst, and vice-chair of the COST action COMULIS correlative multimodal imaging for life sciences.
- Talk title: Correlative microscopies: quantitatively fusing information from 3D+ to 6D+
Dr Daniel Baum (Zuse Institute Berlin, Germany)
Talk title: Visual Data Analysis: Bringing the Human into the Loop
Daniel Baum is head of the research group »Visual Data Analysis« and deputy head of the department »Visual and Data-Centric Computing« at the Zuse Institute Berlin (ZIB), Germany. He studied computer science at the Humboldt-Universität and the University of Edinburgh. In 2007, he received his Dr. rer. nat. degree from the Freie Universität Berlin with a thesis on molecular shape analysis. His current research interests include visual data analysis, computer graphics, image analysis as well as shape modelling and analysis. Daniel has many years of experience in interdisciplinary collaborations with researchers from very different fields like biology, geoscience, material science and ancient studies. He therefore regularly works on data of different origin, size, and scale from a variety of image sources, including micro-computed tomography, electron tomography, but also histological data and 2-photon microscopy. As part of his research, for almost 20 years, Daniel has been an active developer of the Amira software, which was originally developed at ZIB and is now jointly developed with Thermo Fisher Scientific.
- Talk title: Visual Data Analysis: Bringing the Human into the Loop
Software and Smart Microscopy
Session Chair: Dr Siȃn Culley (University College London, UK)
The modern bioimaging pipeline requires analysis throughout the process - from integrating image analysis into acquisition, e.g. smart microscopy, through to software solutions for the range of quantification pipelines necessary to glean new scientific insights from imaging. This session will focus on state-of-the-art software solutions for automated microscopy and quantitative imaging and post-acquisition analysis.
Dr Leila Muresan (Cambridge Advanced Imaging Centre, UK)
Talk title: Computational enhancement of lightsheet microscopy imaging
Leila Muresan works in fluorescence microscopy image analysis, with special focus on solutions for single molecule imaging and lightsheet microscopy. After completing a PhD in Engineering at Johannes Kepler University, Linz (Austria), she continued with post-doctoral stays at ENS (Paris) and CGM-CNRS (Gif-sur-Yvette). Currently, she is an EPSRC Research Software Engineer Fellow in Cambridge Advanced Imaging Centre. She is affiliated with the Department of Physiology, Development and Neuroscience as well as Sainsbury Laboratory (University of Cambridge).
- Talk title: Computational enhancement of lightsheet microscopy imaging
Dr Martin Weigert (EPFL, Switzerland)
Talk title: Image Segmentation with Learned Shape-Representations
Martin Weigert is a computer scientist whose research focuses on computational optics and machine learning based image reconstruction and segmentation for microscopy. He did his PhD at the MPI-CBG in Dresden and recently joined EPFL, Switzerland as a group leader.
- Talk title: Image Segmentation with Learned Shape-Representations
Session Chair: Dr Hannah Nerl (Fritz-Haber Institute of the Max Planck Society, Germany)
Functional materials cannot be studied reliably when removing materials from their reaction environment. Recent operando studies aim to address this by correlating structure and function of materials under working conditions. Significant technical advances in instrumentation have led to the development and improvement of a range of operando techniques with great impact across scientific fields. These operando approaches have already been shown to allow for the visualization and analysis of materials during synthesis, degradation or function in well-defined environments. Aside from electron microscopy, relevant examples of emerging and improved operando techniques include X-ray microscopy, scanning probe microscopy, light microscopy and atomic force microscopy. This session will contain contributed and invited talks and posters that aim to highlight recent technical advances in operando approaches and the resulting science while studying a range of materials including 2D materials, nanoparticles and catalysts.
Dr Irene Groot (Leiden University, Netherlands)
Talk title: Seeing is believing: Atomic-scale imaging of catalysts under reaction conditions
Irene Groot obtained her PhD degree at Leiden University investigating the dissociation of hydrogen on single crystals, both experimentally using supersonic molecular beams and theoretically performing density functional theory and quantum dynamics calculations. After a postdoc at the Fritz Haber Institute in Berlin, where she studied CO oxidation using sanning tunneling microscopy, she went to the Leiden Institute of Physics with a personal Veni fellowship from the Netherlands Organization for Scientific Research. Here she investigated HCl oxidation using scanning tunneling microscopy under reaction conditions, and subsequently started her own group.
Currently, Irene Groot is associate professor (tenured) at the Leiden Institute of Chemistry where she is heading the operando catalysis research group. She investigates the structure-activity relationship of catalysts under industrial conditions using operando scanning probe microscopy, transmission electron microscopy, Raman spectroscopy, and X-ray-based techniques. She focuses on industrial processes related to sustainable energy and materials production. Current topics in her group are hydrodesulfurization, Fischer-Tropsch synthesis, methanol steam reforming, automotive catalysis, chlorine production, and graphene growth on liquid copper. Additionally, she is developing equipment for high-pressure surface science and operando catalysis research.
- Talk title: Seeing is believing: Atomic-scale imaging of catalysts under reaction conditions
Dr Thomas W Hansen (DTU Nanolab, Technical University of Denmark, Denmark)
Talk title: Dynamics of nanostructure surfaces and ways to approach them
Thomas Hansen studied physics at the University of Copenhagen where he received his M.Sc. in 2001 finishing with a project on the use of electron energy-loss spectroscopy for the study of carbon-based materials. He then pursued his PhD in chemistry as a joint project between the University of New Mexico, the Technical University of Denmark and the catalyst company Haldor Topsøe. During his PhD studies, he used environmental transmission electron microcopy to study the sintering of nickel-based steam reforming catalysts under different environments. After receiving his degree in 2006, he continued his research in catalysis at the Fritz Haber Institute in Berlin where he studied complex mixed metal oxides for selective oxidation processes. In 2008, he returned to the Technical University of Denmark, first in a post-doctoral position, and was in 2010 promoted to senior researcher. Thomas now focuses on the study of the dynamics and growth of nanostructures (nanoparticles and 2D materials) by environmental transmission electron microscopy and the application of automated analysis techniques such as convolutional neural networks. His primary interests are the fundamental properties of nanostructures and the use and development of in situ techniques.
- Talk title: Dynamics of nanostructure surfaces and ways to approach them
Advanced Atom Probe Tomography
Session Chair: Professor Michael Moody (University of Oxford, UK)
Underpinned by advances in instrumentation, experimental protocols and increasingly sophisticated data analysis, Atom Probe Tomography (APT) is now an indispensable characterisation tool in laboratories around the world. The improved capabilities have broadened the scope of investigations and increased the depth of achievable atomic-scale insights. This has nucleated entirely new lines of research, including application to a wider range of material systems ( biological, ceramics, functional materials) and the study of degradation of material microstructure subject to extreme conditions (irradiation, corrosion, hydrogen ingress). This session welcomes submissions pertaining to advanced applications of APT and in particular studies incorporating novel uses of complementary microscopy techniques to enable and enhance APT characterisation.
Dr Baptiste Gault (Max-Planck-Institut für Eisenforschung in Düsseldorf, Germany)
Talk title: Pushing the analytical limits of atom probe tomography via cryo-enabled workflows
After a PhD in physics in Rouen (2006) where we developed a new generation of pulsed-laser atom probe microscopes, I worked as a scientist at the ACMM at the University of Sydney from 2007-2009 and 2010-2012 (jointly with ANSTO), and in-between, I was a Marie Curie postdoctoral fellow at the Department of Materials, University of Oxford where I worked on thermoelectric materials. In 2012, I was appointed Assist. Prof. at McMaster University in Canada, but quitted after 6 months. From Dec. 2012 - Dec. 2015, I interrupted my research career, and took on a position of Senior Publisher in the Materials Science group at Elsevier Ltd. in Oxford, looking after the Materials Engineering portfolio. During that time, I became an academic visitor at the Department of Materials, University of Oxford. On 1st January 2016, I became the Group Leader for Atom Probe Tomography at the Max-Planck-Institut für Eisenforschung in Düsseldorf. Since October 2018, I also hold a part-time appointment as Reader at the Department of Materials, Imperial College London. I received the Leibniz Prize 2020 for my work on the development of the pulsed-laser atom probe tomography and help enable the spread of the technique over the past 15 years.
- Talk title: Pushing the analytical limits of atom probe tomography via cryo-enabled workflows
Professor Rachel Oliver (University of Cambridge, UK)
Talk title: Using microscopy to understand and improve green light emitting diodes
Professor Rachel Oliver is Director of the Cambridge Centre for Gallium Nitride. The focus of her research is understanding how the small scale structure of nitride materials effects the performance and properties of devices. She uses expertise in microscopy and materials growth to develop new nanoscale nitride structures which will provide new functionality to the devices of the future. She is also passionate about communicating science to the general public and hence widening participation in science by under-represented groups, particularly women.
- Talk title: Using microscopy to understand and improve green light emitting diodes
Diamond Light Source Sessions
Session Chairs: Professor Angus Kirkland (University of Oxford, UK) and Dr Peijun Zhang (University of Oxford, UK)
Diamond Light Source is the UK’s national synchrotron user facility with 32 beamlines along with two state-of-the-art electron microscopy laboratories (eBIC for cryo-EM and ePSIC for aberrationcorrected transmission electron microscopy). Users at Diamond have access to a wide range of cutting edge experimental techniques in microscopy and spectroscopy across multiple length scales, including X-ray imaging, hard and soft X-ray microscopy, XANES and XRD mapping, tomography , and phase-retrieval techniques such as ptychography and coherent diffraction imaging. These have wide ranging applications in fields from biology, environmental, earth and planetary sciences, to materials science and engineering. As for cryo-EM, eBIC provides cryoEM single particle analysis, cryo-electron tomography, electron crystallography, and cryoFIB/SEM. Through this session we aim to showcase a selection of user studies conducted at Diamond, with an introductory staff presentation briefly discussing some of the capabilities and access routes.
Dr Sean Collins (University of Leeds, UK)
Talk title: Local diffraction at low dose: Nanoscale structural variation in molecular materials
Sean first worked in electron microscopy and microanalysis in 2009 as an undergraduate student at the National Institute of Standards and Technology in Gaithersburg, Maryland (USA) and subsequently in the Department of Chemistry at the University of Michigan, Ann Arbor. He completed his PhD in Materials Science in 2016 at the University of Cambridge, using electron energy loss spectroscopy to examine the optical properties of plasmonic metal nanoparticles. He worked as a post-doc in Cambridge and then from 2017 as the Henslow Research Fellow at Girton College, Cambridge, investigating domain microstructures of metal-organic framework crystals and glasses. In 2019, Sean was appointed as a University Academic Fellow in the Bragg Centre for Materials Research at the University of Leeds, jointly in the Schools of Chemical and Process Engineering and Chemistry. Sean specialises in tomography, spectroscopy, and scanning electron diffraction with experiments at SuperSTEM, the electron Physical Sciences Imaging Centre (ePSIC) at the Diamond Light Source, and the Leeds Electron Microscopy and Spectroscopy (LEMAS) Centre focusing on nanoscale optical properties and crystallography of molecular materials.
- Talk title: Local diffraction at low dose: Nanoscale structural variation in molecular materials
Professor James Naismith (University of Oxford, UK)
Talk title: Looking inside the cell; molecular pathology using electron tomography
Jim Naismith is the first Director of the Rosalind Franklin Institute, the theme lead for Structural Biology at the Institute and Professor at University of Oxford.
Jim grew up in Hamilton, in the west of Scotland attending local state schools. He graduated from Edinburgh in 1989 with a BSc in Chemistry and with PhD from Manchester. He has been a Carnegie Scholar, a NATO fellow and a BBSRC fellow. His lab have won numerous awards including most recently the Tilden Medal. He has spun out GyreOx from his academic lab, been the expert witness in the most consequential ($10bn) biosimiliar patent case in the USA and has been elected to the Council of the Royal Society. Until 2017 he was the Bishop Wardlaw Professor Chemical Biology at St Andrews. He leads the Franklin team who reported the breakthrough in July in using Llama nanobodies to neutralise the COVID19 virus. The team are focussed on getting these agents into the clinic.
- Talk title: Looking inside the cell; molecular pathology using electron tomography
Professor Mary Ryan (Imperial College London, UK)
Talk title: Understanding the Evolution of Nanoporosity in Alloy Systems using X-ray and Electron Techniques
Professor Mary Ryan FREng, FIMMM, FICorr is currently Professor of Materials Science and Nanotechnology, and Vice-Dean for Research in the Faculty of Engineering at Imperial College London. She joined Imperial from Brookhaven National Laboratory in the US where she worked in the Materials-Environment Interactions Group. She graduated from Manchester University with a Joint Honours degree in Mathematics and Physics and was then awarded a PhD in Materials Science, carrying out early work with in situ electrochemical STM to study passivation phenomena in binary alloy systems. She leads a large interdisciplinary group focused on understanding nanoscale materials, and nanoscale interfaces in and between materials and their environments. She has a particular interest in the development of operando approaches and nanoscale methods in synchrotron science.
- Talk title: Understanding the Evolution of Nanoporosity in Alloy Systems using X-ray and Electron Techniques
Session Chair: Professor Gail McConnell (University of Strathclyde, UK)
Late breaking submissions are invited to report the very latest results in microscopy. This abstract deadline is not intended to be merely an extension of the general submission deadline: instead this session provides an opportunity for researchers to present the most exciting recent developments in the field.