Fabrication of ZnO Nanoparticles by Tanacetum umbelliferum Extract for in vitro Evaluation of Antioxidant, Anti-Inflammatory and Antiarthritic activity

Session

Microscopy to Modelling

Authors

Rabia Zahid (1), Hafiz Muhammad Asif (1), Waseem Afzal (1)

Affiliations

1. Islamia university of Bahawalpur Pakistan

Keywords

Nanoparticles, Tanacetum umbelliferum, antioxidant, anti-arthritic, anti-inflammatory.

Abstract text

Among various metal nanoparticles, Zinc oxide nanoparticles (ZnO-NPs) have striking role. Biosynthesis of ZnO-NPs is an eco-friendly and cost-effective process. It has unique chemical and physical properties with numerous biomedical and biological applications, such as drug delivery, bioimaging, biosensing, antioxidant, anti-arthritic, anti-inflammatory and antimicrobial potential. Keeping in mind the immense potential of Zinc oxide nanoparticles, in current study Tanacetum umbelliferum Boiss was used to synthesize Zinc Oxide nanoparticles, and in vitro therapeutic potential was evaluated for antioxidant, anti-arthritic and anti-inflammatory activity. The standard techniques including UV-visible and FTIR spectroscopies followed by SEM, XRD and EDX analysis were employed for the characterization of ZnO-NPs. Sample gave λmax peaks in between 350-360 nm (UV-vis spectra). The Fourier-transform infrared spectroscopy results showed several alcoholic, proteins, flavonoids and phenolic functional groups. Crystalline nature, particle sizes and morphology of the ZnO-Nps were established by the analysis of SEM, XRD, and EDX results. Antioxidant (DPPH radical scavenging activity), anti-inflammatory (egg albumin denaturation method) and anti-arthritic activity (inhibition of protein denaturation method) were performed by ZnO-NPs. As lower IC90 reflects higher percentage inhibition of antioxidant (36.36), anti-arthritic activity (36.51) and higher IC90 showed lower anti-inflammatory activity (49.93). It has been proven that Zinc oxide nanoparticles of T. umbelliferum are active, safe and potent option for management of arthritic disorders such as gout.

References