Stimulated emission depletion (STED) microscopy has proven to be an imaging technique, which provides its’ users with the possibility of generating high-quality images with a resolution down to 20 nm.For example the STEDYCON, which is a completely new class of nanoscope, converts your conventional epifluorescence microscope into a versatile four-colour confocal and STED system. I will also talk about the features of the Facility Line, combining cutting-edge microscopy with unprecedented ease-of-use including technologies like adaptive optics and the novel MATRIX detector.Finally, I will explain the novel VAHEAT system from Interherence, which is a precise temperature control unit for optical microscopes. It combines local heating with direct temperature sensing in the sample volume.

The pco.flim is the first Frequency Domain FLIM camera using a two tap CMOS sensor. Synchronized modulation of pixels and stimulated light enables you the analysis of luminescence decay times in the range of 100 ps – 100 µs. With its 1008 x 1008 pixels resolution the pco.flim reads out 45 double frames/s at a max. You can use it for a modulation frequency range of 5 kHz – 40 MHz. Using C-mount as standard the system is easy to connect to any microscope or lens. Further, the USB 3.0 interface lets you connect the camera to all kinds of computers. This saves you significant efforts and costs for operation and research. This video tutorial explains how fluorescence lifetime imaging (FLIM) can be done with the frequency domain camera and laser system pco.flim.