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Multi-Modal Optical Microscopy with a Fully Integrated Coherent Raman Scattering - Confocal Microscopy Platform: Applications from Cells to Tissues to Model Organisms

Multi-Modal Optical Microscopy with a Fully Integrated Coherent Raman Scattering - Confocal Microscopy Platform: Applications from Cells to Tissues to Model Organisms

Session

Stream 5: Label Free Imaging

Authors

Dr Volker Schweikhard (1)

Affiliations

1. Leica Microsystems CMS GmbH

Keywords

Stimulated Raman Scattering, Coherent Raman Scattering, Label-Free Imaging, Multimodal Imaging

Abstract text

Coherent Raman Scattering (CRS) microscopy, in particular the emerging stimulated Raman scattering modality, provides a rich palette of label-free chemical and biophysical contrast mechanisms that are beginning to find a wide range of applications from the life sciences, to basic and preclinical medical research, and to materials research. The vibrational signals are quantifiable and largely orthogonal to fluorescent signals from the same specimen. A number of additional nonlinear optical signals an often be acquired in parallel, including second harmonic generation and multi-photon fluorescence. For these reasons, a full integration of Coherent Raman Scattering into a flexible and versatile confocal microscopy platform provides unprecedented opportunities for correlative multimodal optical imaging. My presentation will include a primer on the physical background of coherent Raman scattering, and showcase a wide range of application examples of multi-modal imaging with vibrational contrast, ranging from cell and tissue biology to imaging deep inside intact small model organisms.

References