Multiscale imaging at high spatiotemporal resolution

Session

Optical Imaging of Fast Dynamic Processes

Authors

Dr. Hari Shroff (1)

Affiliations

1. Howard Hughes Medical Institute

Keywords

super-resolution microscopy; 3D SIM; deep learning; image restoration

Abstract text

I will describe two recent efforts to improve the imaging of living specimens at different spatiotemporal scales. First, I will discuss improvements to the axial resolution of 3D structured illumination microscopy (3D SIM), a widely used super-resolution technique appropriate for imaging single cells [1]. By incorporating a mirror opposite the sample, we are able to improve axial resolution ~2-fold over 3D SIM (or 4-fold over widefield microscopy), producing super-resolution images with near-isotropic resolution. We also demonstrate a complementary method that harnesses deep learning to achieve isotropic images directly from noisy 3D SIM images, which is particularly beneficial for volumetric time-lapse (4D) imaging. Second, I will discuss multi-view confocal microscopy [2], a method that improves the resolution of confocal microscopy and can similarly be combined with deep learning to provide class-leading performance in thicker, scattering samples. The tradeoffs as well as advantages of both methods will be discussed candidly with an eye to future improvements. 

References

1) Li, X., Wu, Y., Su, Y., Rey-Suarez, I.A., Matthaeus, C., Updegrove, T.B., Wei, Z., Zhang, L., Sasaki, H., Li, Y., Guo, M., Giannini, J.P., Vishwasrao, H.D., Chen, J., Lee, S-J. J., Shao, L., Liu, H., Ramamurthi, K.S., Taraska, J.W., Upadhyaya, A., La Riviere, P., Shroff, H. Three-dimensional structured illumination microscopy with enhanced axial resolution. Nature Biotechnology, available online (2023).

2) Wu, Y., Han, X., Su, Y., Glidewell, M., Daniels, J.S., Liu, J., Sengupta, T., Rey-Suarez, I., Fischer, R., Patel, A., Combs, C., Su, J., Wu, X., Christensen, R., Smith, C., Bao, L., Sun, Y., Duncan, L.H., Chen, J., Pommier, Y., Shi, Y-B., Murphy, E., Roy, S., Upadhyaya, A., Colón-Ramos, D., La Riviere, P., Shroff, H. Multiview confocal super-resolution microscopy. Nature 600 (7888): 279-284 (2021).