Integration of Mass Spectrometry Imaging with MeV Ion Beam Analysis to Enable Visualisation of Lipids and Biologically Relevant Elements in a Single Tissue Section
- Abstract number
- 467
- Presentation Form
- Contributed Talk
- Corresponding Email
- [email protected]
- Session
- Mass Spectrometry Imaging Across Length Scales in Life and Physical Sciences - Providing Atomic and Molecular Information
- Authors
- Melanie Bailey (2), Catia Costa (2), Johanna von Gerichten (2), Janella de Jesus (1), Josephine Bunch (1)
- Affiliations
-
1. National Physical Laboratory
2. University of Surrey Ion Beam Centre
- Abstract text
The colocation of elemental species with host biomolecules such as lipids and metabolites may shed new light on the dysregulation of metabolic pathways and how these affect disease pathogeneses. For example, alkali metals have been the subject of extensive research, are implicated in various neurodegenerative and infectious diseases and are known to disrupt lipid metabolism. Desorption electrospray ionisation (DESI) and secondary ion mass spectrometry (SIMS) are widely used approaches for molecular imaging, whereas MeV Ion Beam Analysis can be used to image elements in biological samples at 1 micron resolution. Here we show some of the challenges and successes of co-locating metals and lipids in tissues, including sample handling, analysis workflows and data integration.