Double Helix Optics delivers 3D microscopy with unparalleled depth and nano-scale precision. Through their Engineered Point Spread Function-based technology, conventional 2D imaging is easily extended into the third dimension, enabling scan-free 3D localization, 4D particle tracking, and extended depth of field. DHO’s SPINDLE line of products, library of phase masks, and 3DTRAX software produce a robust and versatile solution for capturing more information from existing microscopy setups.