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  • X-ray microscopy for deep and precise bioimaging

    Abstract number
    327
    Corresponding Email
    [email protected]
    Session
    Stream 3: X-ray Microscopy: A Powerful Tool to Aid the Understanding of Structures in the Life and Physical Sciences
    Authors
    Alexandra Pacureanu (1)
    Affiliations
    1. ESRF, the European Synchrotron
    Keywords


    Abstract text

    Hard X-ray microscopy is emerging as a disruptive technology for 3D bioimaging at cellular and sub-cellular scales. Recent developments in coherent microscopy approaches, such as X-ray holography using high brilliance nanoprobes, are making possible to explore the 3D structure of tissues and cells with spatial resolutions better than 100 nm. Hard X-rays can penetrate through milliliter-sized tissues and the phase contrast offers exceptional sensitivity in both unstained and stained samples. Cryogenic imaging enables probing the specimens close to their native state and makes possible to reach higher spatial resolutions in radiation sensitive samples. Complementary to phase contrast microscopy, X-ray fluorescence maps the chemical composition of specimens with sensitivity down to ppm level. At beamline ID16A of ESRF, these two techniques are integrated within the same instrument enabling multi-modal measurements of the same samples. Moreover, X-ray holography is compatible with optimal sample preparation for electron microscopy, thus facilitating correlative imaging. I will present recent advances and life sciences applications of X-ray holographic tomography, X-ray fluorescence microscopy and correlative X-ray and electron microscopy.