Physical Virology with Atomic Force Microscopy
- Abstract number
- 265
- DOI
- 10.22443/rms.mmc2021.265
- Corresponding Email
- [email protected]
- Session
- Stream 4 (AFM): Quantitative SPM for Biology, Biomedicine, and Bioinspired Technologies
- Authors
- Dr. Pedro J. de Pablo (1)
- Affiliations
-
1. Universidad Autónoma de Madrid
- Abstract text
The basic architecture of a virus consists of the capsid, a shell made up of repeating protein subunits, which packs, shuttles and delivers their genome at the right place and moment. Viral particles are endorsed with specific physicochemical properties which confer to their structures certain meta-stability whose modulation permits fulfilling each task of the viral cycle. These natural designed capabilities have impelled using viral capsids as protein containers of artificial cargoes (drugs, polymers, enzymes, minerals) with applications in biomedical and materials sciences. Both natural and artificial protein cages (1) have to protect their cargo against a variety of physicochemical aggressive environments, including molecular impacts of highly crowded media, thermal and chemical stresses, and osmotic shocks. Viral cages stability under these ambiences depend not only on the ultimate structure of the external capsid, which rely on the interactions between protein subunits, but also on the nature of the cargo. During the last decade our lab has focused on the study of protein cages with Atomic Force Microscopy (AFM). We are interested in stablishing links of their mechanical properties with their structure and function. In particular, mechanics provide information about the cargo storage strategies of both natural and virus-derived protein cages (2,3,4). Mechanical fatigue has revealed as a nanosurgery tool to unveil the strength of the capisd subunit bonds (5).
- References
[1] Llauró et al. Nanoscale, 2016, 8, 9328.
[2] Hernando-Pérez et al. Small, 2012, 8, 2336.
[3] Ortega-Esteban et al. ACS Nano, 2015, 9, 10826, ACS Nano, 2015, 9, 10571.
[4] Jiménez-Zaragoza et al. (2018) https://doi.org/10.7554/eLife.37295.001
[5] Martín-González et al. Nucleic Acids Reseach 2019, (47) 9231.