Phototoxicity Workshop - Friday 9 July
Scientific Organisers: Dr Philippe Laissue, Dr Claire Brown and Professor Maddy Parsons
14.00 - 17.00 hours BST
In fluorescence microscopy, phototoxicity describes the phenomenon by which the light used for fluorescence excitation leads to physiological changes in the observed living sample. These changes may go unnoticed or may damage the sample - and can lead to erroneous conclusions drawn from the experiment. In this workshop, we give participants an overview of the problem of phototoxicity. We aim to first give you the basics to better understand phototoxicity - from useful definitions (phototoxicity, intensity, exposure etc.) to key concepts. We then discuss some concrete approaches for assessing, minimising and reporting phototoxicity in different live imaging experiments. As this a very broad area - with a challenging diversity of different biological model systems (e.g. cells, small organisms, plants...), fluorescent probes and microscope modalities - we include many references to relevant literature. We end with a summary of key considerations - and hope to have ample time for engaging discussions!
Invited Speakers will include:
This workshop is free to attend, you can add it to your schedule when registering to attend.